PRODUCT INFORMATION

 I.TECHNICAL SPECIFICATIONS

Structural / Composition Data

Sterility

 Non-sterile

Form

 Powder

Shelf life

 Limited shelf life, expiry date on the label

Composition

-Tryptone: 10 g/L
-Yeast extract: 1 g/L
-Potassium dihydrogen phosphate: 3 g/L
-Dipotassium hydrogen phosphate: 4.8 g/L
-Ammonium sulphate: 3 g/L
-Magnesium sulphate heptahydrate: 0.2 g/L
-L-Cysteine HCl monohydrate: 0.5 g/L
-Sodium propionate: 15 g/L
-Lactose: 7.4 g/L
-Galactose: 2.6 g/L
-Agar: 15g/L

Application

-M-17 Broth is a selective medium.
-Specifically designed for the cultivation of Streptococcus thermophilus.
-Widely used in yogurt testing.
-Applied for the enrichment of S. Thermophilus in the dairy industry.

Preparation

Suspend 54.7 g of dehydrated medium in 950 ml of distilled water (use 1 liter for non-selective medium). Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 115 °C for 15 minutes.
For selective isolation of Bifidobacteria, add the contents of two 25 ml vials of Lithium Mupirocin Supplement. Mix carefully to avoid air bubble formation and dispense as desired.

Note: This medium is sensitive to heat; excessive heat treatment may adversely affect its properties.

Manufacturer- tradename 

 Polygon scientific

Technique

 Microbiological culture: suitable

Ph

 6.7±0.2 (25 °c)

Storage temperature

 2-25°c

Suitability

Bifidobacterium spp.

 II. APPLICATIONS

Intended Uses

-Food and beverages
-Microbiology

 IV. PACKAGING & PRESENTATION

Sizes

100 mg

500 mg

1000 mg