M17 Broth

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M-17 Broth is a specialized medium formulated for the selective cultivation of Streptococcus thermophilus in yogurt testing. It efficiently supports lactic streptococci, including S. cremoris, S. diacetilactis, and S. lactis, while enabling isolation of lactose-deficient mutants. Its nutrient-rich base with lactose and supplements promotes streptococcal growth, stabilizes pH, and restricts lactobacilli proliferation.

Basic, powder, for Streptococcus thermophilus

PRODUCT INFORMATION

 

 I.TECHNICAL SPECIFICATIONS

Structural / Composition Data

Sterility

 Non-sterile

Form

 Powder

Shelf life

 Limited shelf life, expiry date on the label

Composition

 -Ascorbic acid:0.5 g/L
 -Lactose:5 g/L
 -Magnesium sulfate: 0.25 g/L
 -Meat extract:5 g/L
 -Meat peptone (peptic):2.5 g/L
 -Sodium glycerophosphate:19 g/L
 -Soya peptone (papainic):5 g/L
 -Tryptone:2.5 g/L
 -Yeast extract:2.5 g/L

Application

-M-17 Broth is a selective medium.
-Specifically designed for the cultivation of Streptococcus thermophilus.
-Widely used in yogurt testing
-Applied for the enrichment of S. Thermophilus in the dairy industry.

Preparation

 Suspend 42 g of the medium in 1 liter of distilled water and allow it to hydrate. Heat to boiling, 
distribute into   appropriate containers, and sterilize by autoclaving at 121 °C for 15 minutes.

Manufacturer- tradename 

 Polygon scientific

Technique

 Microbiological culture: suitable

Ph

 7.0±0.2 (25 °c)

Storage temperature

 2-25°c

Suitability

-Streptococcus thermophilus
-Nonselective for Enterococcus spp.
-Nonselective for Staphylococcus spp.

 

 II. APPLICATIONS

Intended Uses

-Food and beverages
-Microbiology

 

 IV. PACKAGING & PRESENTATION

Sizes

100 mg

500 mg

1000 mg

 

M17 Broth

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